Plasmid
pCeBiTec18

Part:BBa_K2638560:Design

Designed by: Levin Joe Klages   Group: iGEM18_Bielefeld-CeBiTec   (2018-09-02)


New designed control vector with eCFP for the measurement of the expression strength


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2973
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2973
    Illegal NheI site found at 896
    Illegal NheI site found at 919
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2979
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2973
    Illegal XhoI site found at 1957
    Illegal XhoI site found at 2849
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2973
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2973
    Illegal XbaI site found at 2988
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Oligonucleotides



Source

Biobrick


References